変性セルロース誘導体を用いたスプレードライヤー法による生体触媒の固定化

概要

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Immobilized Saccharomyces cerevisiae was prepared with a modified cellulose derivative which is soluble in high pH solutions and insoluble in low or neutral pH solutions. First, S. cerevisiae cells were mixed in a solution of the cellulose derivative, which was adjusted to high pH with ammonia. Then, ammonia and water in the mixture were vaporized with a spray-dryer. Since the pH of the mixture became neutral, the cellulose derivative formed an insoluble complex with the yeast cells. At the same time, the insoluble complex was dried with the spray-dryer. Consequently, immobilized yeast particles could be obtained in a one-step operation. The production of ethanol from sucrose using the immobilized yeast in a batch fermentation was confirmed. The immobilized yeast was also able to invert sucrose in a continuous reaction system.
社団法人日本生物工学会の論文
1994-11-25