遺伝子工学の基盤技術開発

概要

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In applied microorganisms, improvement of producing strains by sequential mutation and breeding of new strains by introduction of interesting genes have brought technological innovation. It was in 1966 that the author initiated his work on the use of microorganisms to convert chemical compounds to bio-compounds, a field which has recently been called green chemistry, and he has thus worked on in vivo and in vitro genetic engineering more than 35 years. Here, the author summarizes his group's work on the following six technological developments: (1) quantitative assay method for ribosomal RNA; (2) in vivo genetic manipulation with thermo-inducible PldrlOOKM and RP4::Muc£s to expand host bacteria from Escherichia coli to all gram-negative bacteria; (3) expression vectors in E. coli, Bacillus subtilis, lactic acid bacteria and propionic acid bacteria and production of useful bioactive proteins; (4) wide range promoterproving vectors in gram-negative bacteria and B. subtilis; (5) host-vector systems in Klebsiella, Streptomyces, Nocardia, Clostridium, Erwinia, Xanthomonous, Acetobacter, Rhizobium, Lactobacillus, Propionibacterium species and their applications; (6) symbiotic engineering and its application to phytoremediation and to breeding of industrial plants.
2004-01-25