炎光光度検出ガスクロマトグラフィーによる生体試料中タウリンの定量

概要

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A selective and sensitive method for the determination of taurine by GC is described. Taurine was converted into its N-isobutoxycarbonyl dibutylamide derivative by the procedure reported previously {Bunseki Kagaku, 34 128 (1985)}, and analyzed by GC with flame photometric detection (FPD-GC) using DB-1 capillary column. By FPD-GC, a linear calibration curve was obtained in the range of 0.2〜5.0 nmol of taurine, and the detection level was about 20 times more sensitive than that obtained by GC with flame ionization detection. Taurine in biological sample could be measured without any influence of coexistent substances. The recoveries of taurine added to mouse tissues and biological fluids were 96〜107% and the reproducibility was found to be satisfactory. Analytical results of taurine contents in mouse tissues during growth are also presented.
社団法人日本分析化学会の論文
1989-08-05