Comparison of Retention Times of Polypeptides in Reversed Phase High Performance Liquid Chromatography on Polystyrene Resin and on Alkyl Bonded Silica (生体成分の高速液体クロマトグラフィ-)

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概要

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• Retention times were estimated for eleven-tryptic peptides of calmodulin, calmodulin and S100 protein on the various reversed phase under the same elution condition, that is, linear gradient system from 20 % (v/v) to 75 % acetonitrile in 0.1 % (v/v) trifluoroacetic acid. The comparison of retention times was performed on the various combinations of the columns: Polystyrene (Hitachi-Gel 3013) vs. C_<18> silica (Develosil ODS-3), hydroxylmethyl (HO-CH_2-) polystyrene (Hitachi-Gel 3013-O) vs. C_<18> silica (Develosil ODS-3), C_1 silica (TSK gel TMS-250) vs. C_<18> silica (Develosil ODS-3) and C_<18> silica (μ Bondapak) vs. C1 g silica (Develosil ODS-3). The relationships in above combination were linear respectively except for hydroxylmethyl polystyrene.

• 社団法人日本分析化学会の論文
• 1984-07-05

著者

• Fang Yan-il

  Department Of Chemistry Faculty Of Science Tokyo Metropolitan University

• Okuyama Tsuneo

  Department of Chemistry, Faculty of Science, Tokyo Metropolitan University

• KAWASAKI Hiroshi

  Department of Chemistry, Faculty of Science and Engineering, Saga University

• AMANO Yasuhiko

  Department of Chemistry, Faculty of Science, Tokyo Metropolitan University

• Chen Xian-chuan

  Department Of Chemistry Faculty Of Science Tokyo Metropolitan University

• Amano Yasuhiko

  Department Of Chemistry Faculty Of Science Tokyo Metropolitan University

• Okuyama Tsuneo

  Department Of Chemistry Faculty Of Science Tokyo Metropolitan University

• KUROSU^[○!R] Yasuyuki

  Department of Chemistry, Faculty of Science, Tokyo Metropolitan University

• ISOBE Toshihiko

  Department of Chemistry, Faculty of Science, Tokyo Metropolitan University

• Isobe Toshihiko

  Department Of Chemistry Faculty Of Science Tokyo Metropolitan University

• Kurosu^ Yasuyuki

  Department Of Chemistry Faculty Of Science Tokyo Metropolitan University

• Kawasaki Hiroshi

  Department Of Allergy And Rheumatology Research Hospital Institute Of Medical Science University Of

• Kawasaki Hiroshi

  Department Of Chemistry Faculty Of Science Tokyo Metropolitan University

• Kurosu Yasuyuki

  Department of Chemistry, Faculty of Science, Tokyo Metropolitan University

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