High Performance Ion Exchange Chromatography of Proteins (生体成分の高速液体クロマトグラフィ-)
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概要
- 論文の詳細を見る
High performance ion exchange chromatography using aqueous solvents is presented for the separation of proteins. Diethylaminoethyl(DEAE) resin for the anion exchange column and sulfopropyl(SP) resin for the cation exchange column were utilized. Proteins, having a molecular weight of 10000-190000 daltons and an isoelectric point of 3.9-10.7, were separated within 60 min by sodium chloride linear gradient elution. The protein was eluted according to its isoelectric point. These columns are capable of separating proteins with the biological activity in high speed, high resolution, large capacity and considerably high recovery.
- 社団法人日本分析化学会の論文
- 1984-07-05
著者
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Isobe Toshiaki
Department Of Chemistry Tokyo Metropolitan University
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Isobe Toshiaki
Department Of Chemistry Faculty Of Science Tokyo Metropolitan University
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Okuyama Tsuneo
Department of Chemistry, Faculty of Science, Tokyo Metropolitan University
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KADOYA^[○!R] Toshihiko
Department of Chemistry, Faculty of Science, Tokyo Metropolitan University
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AMANO Yasuhiko
Department of Chemistry, Faculty of Science, Tokyo Metropolitan University
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KATO Yoshio
Central Research Laboratory, Toyo Soda Manufacturing Co.,Ltd.,
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NAKAMURA Koji
Central Research Laboratory, Toyo Soda Manufacturing Co.,Ltd.,
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Amano Yasuhiko
Department Of Chemistry Faculty Of Science Tokyo Metropolitan University
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Okuyama Tsuneo
Department Of Chemistry Faculty Of Science Tokyo Metropolitan University
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Nakamura Koji
Central Research Laboratory Toyo Soda Manufacturing Co. Ltd.
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Kadoya^ Toshihiko
Department Of Chemistry Faculty Of Science Tokyo Metropolitan University
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Kato Yoshio
Central Research Laboratory Toyo Soda Manufacturing Co. Ltd.
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Kadoya Toshihiko
Department of Chemistry, Faculty of Science, Tokyo Metropolitan University
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