Simultaneous Determination of Homovanillic Acid,Vanillylmandelic Acid and 5-Hydroxyindole-3-acetic Acid in Urine by High Performance Liquid Chromatography (生体成分の高速液体クロマトグラフィ-)

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概要

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• A simple and reliable method is described for the simultaneous determination of the biogenic amine metabolites homovanillic acid, vanillylmandelic acid and 5-hydroxyindole-3-acetic acid in human urine. The metabolites in urine were cleaned up by chromatography on a column of QAE-Sephadex A-25 and organic extraction. They were separated by isocratic elution high performance liquid chromatography on a reversed phase column. Detection and quantification were achieved with both ultraviolet and electrochemical detection. The correlation between the detection methods was high. The coefficients of variation of the determination in both within-day and between-day were 5-10 % for about 1.5 mg of each acid per liter urine.

• 社団法人日本分析化学会の論文
• 1984-07-05

著者

• Yamaguchi Yuko

  Central Research Laboratory Mitsubishi Petrochemical Co.

• Tamura Zenzo

  Faculty Of Pharmaceutical Sciences University Of Tokyo

• Tamura Zenzo

  Faculty Of Pharmaceutical Sciences University Of Tokyo:(present Address)keio University Hospital Pha

• YOSHIDA Akira

  Central Research Laboratory, Mitsubishi Petrochemical Co.,

• YOSHIOKA^[○!R] Masanori

  Faculty of Pharmaceutical Sciences, University of Tokyo

• Yoshioka^ Masanori

  Faculty Of Pharmaceutical Sciences University Of Tokyo:(present Address)faculty Of Pharmaceutical Sc

• Yoshida Akira

  Central Research Laboratory Mitsubishi Petrochemical Co.

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• Simultaneous Determination of Homovanillic Acid,Vanillylmandelic Acid and 5-Hydroxyindole-3-acetic Acid in Urine by High Performance Liquid Chromatography (生体成分の高速液体クロマトグラフィ-)
• ANALYSIS OF CHINOFORM BINDING TO HUMAN SERUM ALBUMIN BY AN IMPROVED PARTITION EQUILIBRIUM METHOD
• Automatic Analysis of Homovanillic Acid and Vanilmandelic Acid by Anion Exchange High Performance Liquid Chromatography (生体成分の高速液体クロマトグラフィ-)
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