パーミエーションチューブ-GC法による生体試料用ガス分析装置の試作(II)

概要

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Prior to analysis of blood gases, CO_2 and 0_2, a simulation experiment was carried out with standard samples known in their concentrations to testify reliability of an equation expressed as C=R.E./P.R.+C.T. (C : concentration, R.E. : regression equation between concentration and peak area, P.R. : permeation rate, C.T. : correction term). The relative errors in the measurements to the standard samples were 5.17% for O_2 and 8.34% for CO_2. The errors were reduced to 2% for both gases within a normal range of their concentrations in blood. This result proved satisfactory analysis of blood gases with the present method. Determinations of total 0_2 and C0_2 in blood were then performed with 100 microliter of heparinized blood. Lactic acid was used as a gas generating material for the determination of total C0_2 and a mixture of K_3Fe(CN)_6 and saponin(1 + 1) for total O_2. The coefficients of variation were 2.75% and 1.98% for the total O_2 and CO_2, respectively. The good reproducibility assured an application of the present method to blood sample. Samples were analyzed in about 15 minutes, that is, approximately 10 minutes shorter than with commonly used Astrup's apparatus. The correlation between both methods, PT-GC and Astrup's, was very good(r_<O_2>=0.932, r_<C0_2>=0.973).
社団法人日本分析化学会の論文
1976-12-10