ホルムアルデヒドによるインドール-3-酢酸のけい光分析

概要

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When an acid solution is added to a mixed solution of IAA and formaldehyde, the solution fluoresces. The mixed solution in hydrochloric acid showed two excitation peaks at 330 nm and 380 nm, and only a single fluorescence peak at 490 nm. In this work, the optimum conditions, and interferences have been examined. From the result, the following analytical procedure is recommended: One ml of solution containing 1xlO^<-5>〜5x10^<-7>mol/l of IAA and 1 ml of formalin (containing 37% formaldehyde) is added to 2 ml of 1 N HCl. After standing for 45 min. at room temperature, the intensity of fluorescence at 490 nm (excited at 330 nm) is measured. In this method, many kinds of diverse indole compounds interfered even in the same amount of IAA, while inorganic ions except Fe^<2+>, Hg^<2+>, and I-did not interfere.The calibration curve was linear in the range of about 1x10^<-5>〜5×10^<-7> mol/l {(1.8〜O,1)μg/ml} of IAA.
社団法人日本分析化学会の論文
1975-10-10