マトリックス支援レーザー脱離イオン化飛行時間型質量分析法によるハプテン-ウシ血清アルブミン及び酵素-抗体複合体の分析(<特集>質量分析法の応用技術)

概要

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The molecular weights of hapten-BSA conjugates were rapidly determined by MALDI-TOFMS. Forskolin, deacetylforskolin and etoposide were the haptens used to prepare the hapten-BSA cojugates. Protonated molecules ([M+H]^+) of these cojugates were detected clearly by MALDI-TOFMS, and therefore, the number of haptens molecules bonded to BSA could be calculated easily. The relationship between the number or hapten molecules bonded to BSA and the immune response was studied. Enzyme-antibody conjugates were also determined by MALDI-TOFMS. In the HRPO-SOD antibody (IgG) conjugate, the ion of m/z 240000 was detected ; this ion corresponds to the conjugate of three moles of HRPO bonded to IgG. There was a linear relationship between the number of HRPO molecules conjugated to IgG and ELISA activity.
社団法人日本分析化学会の論文
1996-06-05