液体クロマトグラフィー : タンデム型質量分析法によるヒト血清中26,26,26,27,27,27-ヘキサフルオロ-1α, 25-ジヒドロキシビタミンD_3の定量分析(<特集>質量分析法の応用技術)

概要

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A highly sensitive and specific liquid chromatographic/tandem mass spectrometric method was developed for the determination of 26,26,26,27,27,27-hexafluoro-dihydroxy-vitamin D_3,a new fluoro analogue of 1.25 (OH)_2-vitamin D_3. It was found that this compound gave an acetic an acetic acid-adducted ion with high intensity when the mobile phase contained acetic acid. By optimizing the mobile phase composition in the liquid chroma-tography system in obtaining the highest response of the adduct ion, this compound in serum was determined down to as low concontration as 0.1 pg/ml, following only deproteinization by acetonitrile and purification on solid phase cartridge. It was recognized that the present assay method has sufficient precision, rapidity and simplicity in execution, and enables us to carry out the determination of very low concentration of this compound in clinical studies for humans.
社団法人日本分析化学会の論文
1996-06-05