内標準を用いるガスクロマトグラフィー : 質量分析法による尿中チミングリコールの定量に基づくカテキン及びルチンの活性酸素消去能の評価 (<特集>健康と分析化学)

概要

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Mass spectrometry using deuterium-labeled standard was applied to determine thymine glycol in the urine of rats fed with catechin and rutin in order to evaluate their anfioxidative efficacy. The rats were freely fed a 20% casein diet containing 1% catechin and/or 1% rutin and water for 56 days. The urine samples were collected on the last 2 days of feeding. Thymine glycol-d_3 was added to one ml aliquots of the urine samples, and then recovered with thymine glycol in the samples by affinity chromatography, solid-phase extraction and HPLC using an ODS column. The recovery of thymine glycol was 40-56% through the purification steps. The thymine glycol fractions recovered were converted to tetra-TMS derivatives and then applied to GC/MS analysis. Thymine glycol in urine samples of rats fed with catechin decreased to 68% levels (P < 0.05) of those of no-administered control. However, the antioxidative efficacy of rutin was not observed by this method. These results suggest that catechin has the ability to prevent oxidative DNA damage on the basis of thymine glycol levels in rat urine samples.
社団法人日本分析化学会の論文
2000-06-05