新規添加剤を用いるキャピラリー電気泳動法における分離能改善の研究
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概要
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Studies on capillary electrophoresis (CE) were attempted in order to develop novel and simple migration systems to control the electroosmotic and electrophoretic mobility and to improve the resolution. First, a zwitterionic surfactant (Zwittergent-3-14) was very useful to suppress the adsorption of proteins to the capillary wall and to control the electrophoretic mobility of inorganic anions by modifying the electroosmotic flow. This system was successfully applied to the determination of ionic components in real samples as follows (1) a vitamin (biotin) in a tablet, (2) NO_2^-, NO_3^-, and SCN^- in human saliva samples, and (3) Br^- in seawater. Second, some charged cyclic compounds were examined as CE additives for the separation of structural and positional isomers. Negatively charged cyclodextrin (sulfonated-β-cyclodextrin) was effective in the separation of several alkylphenols and bisphenols. Under the optimized CE condition, the structural isomers of nonylphenol could be successfully distinguished by more 20 individual peaks. Cationic diazacrown ethers having quaternary ammonium ions were useful as CE additives to improve the resolution among the positional isomers of divalent aromatic anions. The ion association constants between the cationic diazacrown and analyte anions were evaluated and the separation mechanism was considared. These studies using several novel additives provided an improvement in the peak efficiency, the peak resolution and the peak reproducibility in CE separation.
- 社団法人日本分析化学会の論文
- 2002-03-05
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- 新規添加剤を用いるキャピラリー電気泳動法における分離能改善の研究