IMMUNOCHEMICAL METHOD FOR DETERMINATION OF △^1-PYRROLINE

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概要

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• An enzyme immunoassay for △^1-pyrroline after being converted to 2,3-trimethylene-4-quinazolone (TQ) through a reaction with o-amino-benzaldehyde followed by chromic acid oxidation is described. The method for determination of TQ is based on chemiluminescence derived from luminol and hydrogen peroxide in the presence of horseradish peroxidase-labeled 6-(β-carboxypropionyl) amino-2,3-trimethylene-4-quinazolone bound competitively to an immobilized anti-TQ antibody on Sepharose 4B. As little as 10 picomole of △^1-pyrroline was successfully determined by this method. The method is applicable to determine some compounds from which △^1-pyrroline is quantitatively liberated by a specific chemical or enzymatic reaction. An example of application is shown for determination of spermidine coupled with spermidine dehydrogenase from Serratia marcescens.

• 1983-03-05

著者

• Samejima Keijiro

  Tokyo Biochemical Research Institute

• Sakamoto Shunji

  Tokyo Biochemical Research Institute

• Shibahara Noriko

  Tokyo Biochemical Research Institute

関連論文

• ^N-ENRICHED POLYAMINES : SYNTHESES AND APPLICATION FOR STUDIES ON POLYAMINE METABOLISM
• Spermidine and Spermine Contents in Foodstuffs
• IMMUNOCHEMICAL METHOD FOR DETERMINATION OF △^1-PYRROLINE
• ENZYME IMMUNOASSAY OF Δ^1-PYRROLINE

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