6.Caの吸収および輸送にたいするDの影響

概要

論文の詳細を見る
NTS cell-line are of a pure colonal clone separated from mass culture cells of newborn rat cerebellum which were maintaining in continuous cultivation since 54 months. Biochemical analysis of NTS cell-line revealed some difference in the quantitative ratio among free amino acids. Immunological tests with the serum antibodies from the sensitized rabbit disclosed that NTS cells have the similar protein components as the rat brain tissue. This cell-line was cultured in such a way that this appeared to be dependent upon vitamin B_<12>. The growth curve of this cell-line was dropped in the methionine- and choline-defficient medium, but this growth was enhanced only when the vitamin B_<12> was added in such defficient medium. The terminal reaction in methionine biosynthesis involves the transfer of a methyl group from 5-methyltetrahy-drofolate to homocysteine. The levels of 5-methylterahydrofolate-homocysteine transmethylase activities were elevated some 10-30 fold on NTS cells in the presence of methyl-B_<12> and homocysteine substituted for methionine and choline in the medium. Elevated enzyme levels were also obseved in primary culture of rat cerebellum maintained neurons and neuroglia cells in above medium. It is assumed above results that the methionine biosynthesis in the nervous tissue cells is required directly the vitamin B_<12>.
日本ビタミン学会の論文
1970-05-25