尿中リポ酸様活性物質について : (II)精製とその化学的性状

概要

論文の詳細を見る
Main biologically active lipoic acid-like substances, which are tentatively named as S_1- and S_2-compounds, were isolated from the human urine following the administration of lipoamide. Both compounds were separated from lipoic acid and other lipoic acid-like substances in the urine by extraction with organic solvents, treatment with ion exchange resins and silica gel column chromatography. It was confirmed that the S_1- and S_2-compounds are derived from ^<35>S-lipoic acid administered to rats. Further, S_2-compound which was larger than S_1,was separated from the mixture by paper chromatography. It responded to Corynebacterium bovis but not Streptococcus faecalis and easily converted to lipoic acid by acid hydrolysis. At the same time it released four ninhydrin-positive compounds on paper chromatogram. By paper electrophoresis the S_2-compound migrated to the anode easier than lipoic acid at a range of pH 3-4. It was also found not to be a simple lipoamide derivative because of stability to lipoamidase which was partially purified from Corynebacterium bovis. The chemical structure of S_2-compound was discussed.
日本ビタミン学会の論文
1969-09-25