L-アスコルビン酸の分解に関する研究 : (VIII)細菌によるL-アスコルビン酸の分解機構 : (3)L-アスコルビン酸分解酵素系におよぼす各種阻害剤およびビタミンの影響

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As preliminary research to clarify the metabolic pathway of L-ascorbic acid by micro-organism, the enzyme system concerning with the decomposition of L-ascorbic acid was investigated by experiments using inhibitors for various enzyme system. As the results of experiments, dehydrogenase system, enolase, Fe-containing enzyme, oxidative decarboxylation, and oxidative phosphorylation were presumed to participate in the decomposition of L-ascorbic acid by inhibition with ICH_2COOH(10^<-1>〜10^<-3>M), NaF(10^<-2>〜10^<-3>M), KCN(10^<-3>M), As_2O_3 (10^<-3>M) and NaN_3(10^<-3>〜l0^<-4>M) to L-ascorbic acid metabolism respectively. Moreover, the existence of TCA-cycle was also suggested by the experiments that resting cells of tested organism had the ability to oxidize some members of TCA-cycle and succinic acid and α-ketoglutaric acid oxidation were inhibited with malonate (10^<-3>M) and As_2O_3(10^<-3>M) respectively. From the above mentioned results, it was supposed that L-ascorbic acid was finally metabolized to CO_2 and H_2O through TCA-cycle. On the other hand, stimulative effects of some vitamins in the L-ascorbic acid metabolism by vitamin-deficiency cells led to the view that these vitamins were concerned with the co-factors of L-ascorbic decomposing enzyme system.
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