Phycomycesを用いるビタミンB_1およびその分解産物の定量 : (I)ビタミンB_1の定量

概要

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The method for thiamine determination using Phycomyces blakesleeanus was tested and the use of a Roux flask instead of Erlenmeyer flask was found preferable as it markedly shortens the incubation time and is more suitable for making many assays at a time. A high blank due to cotton plug as has often been observed in microbiological assays was never observed. The pyrimidine and thiazole moieties of thamine have no effect singly on the growth of the fungus, but it showed about the same effect when used in combination.
日本ビタミン学会の論文