好アルカリ性細菌Bacillus sp.のプロテアーゼ生産に及ぼすpHの影響

概要

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The effect of the pH of the medium on the microbial growth and alkaline proteinase production, and on the uptake of various substances by alkalophilic Bacillus sp. No. 8-1 wre studied to investigate the physiological properties of alkalophilic bacteria. Both the microbial growth and alkaline proteinase production by replacement culture were maximum between pH 9 and 10. The alkaline proteinase production was not affected by the kind of carbon or nitrogen source, in fact, inorganic nitrogen sources were also effective for the production.The uptake of various substances such as glucose, acetate, amino acids, and uracil, necessary for proteinase productioin by this strain, was maximum between pH 9 and 10. The uptake of a-aminoisobutyric acid, a nonmetabolizable amino acid analogue, was also maximum at pH 10. The pH-dependence of these substances was not due to their ionic forms being affected by extracellular pH.It was concluded from above results that good production of alkaline proteinase in alkaline media was due to the active uptake of various nutrients in this culture condition.
社団法人日本生物工学会の論文
1976-08-25