活性汚泥中の粘質物成分としての核酸 : 活性汚泥中の粘質物に関する研究(第2報)

概要

論文の詳細を見る
Gelatinous mucilage of activated sludge was obtained by extracting with an EDTA (Ethylenediaminetetraacetic acid sodium salts) solution (pH 10), followed by precipitation with ethanol.The mucilage was estimated to be composed of protein (20%), nucleic acid (30%) and carbohydrates (7%). Fractionation analysis of nucleic acid by Schmidt. Thannhauser and Schneider's method revealed that the mucilage contained at least 30 per cent of nucleic acid in the activated sludge.Through the extraction procedure, it seems most reasonable to conclude as follows;1. Mucilage containing polymerized nucleic acid is obtained from the sludge by the extraction procedures without destroying the living cells.2. Metallic ion permits the polymerized nucleic acid with protein to be in an insoluble form in the floc.3. Nucleo-protein released by autolysis from the microbial cells is accumulated in the mucilage without further enzymatic degradation.
社団法人日本生物工学会の論文
1974-05-25