Studies on CO_2-fixing Fermentation : (XIV) On the Relationship between L-Malate Formation and Carbohydrate Metabolism of Cell-free Extracts of Schizophyllum commune

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概要

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• Evidences obtained by enzymic studies using crude cell-free extract of Schizo-phyllum commune were as follows.The activity of malic dehydrogenase linked to NAD was strong. The reductive carboxylation of PEP was detected in the presence of Pi, or IDP and KHCO_3.The reductive carboxylation of pyruvate was inhibited by Pi. 3PGA was shown to be more active than pyruvate in the reductive carboxylation system. GAP dehydrogenease activity was detected, and the coupling reaction of malic dehydrogenase with GAP dehydrogenase was found. Aldolase activity was detected, and aldolase could be followed by the reduction of OAA coupled with GAP dehydrogenase.G6P dehydrogenase activity was observed, while it was inhibited by the additions of L-malate and NAD. Both of malic enzyme and lactic dehydrogenase activities could not be found.These evidences indicate that it is affirmative to propose equation (1) and(2)to explaine the process of L-malate fermentation of S. commune.Glucose+2CO_2+4Pi→2_L-malate+2PP…(1)Glucose+2CO_2+2IDP(GDP)+2Pi→2_L-malate+2ITP(GTP)…(2)

• 公益社団法人日本生物工学会の論文
• 1967-12-25

著者

• Tachibana Sei

  Department Of Chemistry Faculty Of Science And Engineering Ritusmeikan University

• Tachibana Sei

  Department Of Chemistry Faculty Of Science And Engineering Ritsumeikan University

• Siode Jiuichi

  Department of Chemistry, Faculty of Science and Engineering, Ritusmeikan University

• Hanai Tosihiko

  Department of Chemistry, Faculty of Science and Engineering, Ritusmeikan University

• Siode Jiuichi

  Department Of Chemistry Faculty Of Science And Engineering Ritusmeikan University

• Hanai Tosihiko

  Department Of Chemistry Faculty Of Science And Engineering Ritusmeikan University

関連論文

• A Spectrophotometrical Study on the Effect of Vitamin B_2-Acid on the Growth of Chattonella antiqua
• Studies on CO_2-fixing Fermentation : (XIV) On the Relationship between L-Malate Formation and Carbohydrate Metabolism of Cell-free Extracts of Schizophyllum commune

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