繊維素分解酵素について : (第1報) 濾紙崩壊によるセルラーゼ活性の測定法ならびに木材腐朽菌およびその他の菌類のセルラーゼ活性

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1. The authors modified Kitamikado-Toyama's method of determination of cellulase activity.This modified method is as follows : Reaction mixture 6 ml (pH 4.0) is taken into an L-shaped glass tube [18-19 mm (diam.), 50 mm (height), 140-145 mm (length)] which is originally a part of Monod's shaking culture apparatus, then 2 sheets (1×1 cm, ca. 25 mg) of filter paper and few drops of toluol are added thereto.At least, 4 or 5 tubes of this type with the same contents as above are prepared for this purpose. They are shaken in a water bath at 40℃ for 150 minutes. After the reaction, the filter paper sheets in each L-shaped tube are transferred into a small sieve [20 mesh, 3×3×2 cm (height)] to be washed in water for 90 minutes. During the washing time, decomposed portions of filter paper are washed out through the sieves. The sieves are dried and weighted. A loss of filter paper mass of each sieve is calculated to find the breakdown percentage of the treated filter paper.2. Activity of cellulase secreted by fungi.The authors cultured Aspergillus sp., wood-destroying fungi and other fungi on the washed wheat bran and extracted enzymes with water from the Koji produced by this culture.The cellulase activity in the extracted liquid was determined by the modified method. Moreover, activities of pectinase, xylanase and tannase contained in the same Koji extract were determined.
社団法人日本生物工学会の論文
1967-11-25