ヒトの血清および尿中のビタミンB_<12>形態ならびにメチルコバラミンの筋肉内投与後の生体内運命, とくに血清および尿中B_<12>の存在形態について

概要

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On investigation of natural occurrence of cobalamin in human serum and urine by chromatographic microbioassay, it was revealed that CH_3・B_<12> in serum, and CH_3-B_<12> and CN-B_<12> in urine were found as the major forms of occurrence. It was confirmed on incubation of urine with ^<57>Co-CH_3・B_<12> that CN・B_<12> fraction was not transformed from CH_3・B_<12> in vitro during the procedure. Following the intramuscular injection of 1,000μg of CH_3・B_<12> to man, the level of cobalamin in the serum apparently increased, amounting up to 33.5 to 53.0 mμg/ml, and after 48 hours it turned back to normal value. As to the excretion of cobalamin in the urine after administration of CH_3・B_<12>, it was found that 34.7 to 43.2% of cobalamin was excreted in the first day and practically no more in the following days. The forms of cobalamin in the serum two hours after the administration of CH_3・B_<12> were found to be CH_3・B_<12> fraction in 80% of total cobalamin activity, and others in fractions of OH-B_<12>, DBCC and CN・B_<12>. The majority of cobalamin activity in the urine 24 hours after the administration were found to be CH_3・B_<12> fraction, but a little activity in OH-B_<12> fraction.
日本ビタミン学会の論文
1970-09-25