纎繊維物質の加水分解と其の醗酵に關する研究 (第11報) : 加水分解物のペニシリン醗酵 : (ii) 各種窒素源による培養試驗とペントーズ液の精製に就て

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Studies on the assimilation of pentoses by Penicillium chrysogenum in connection with the production of penicillin were undertaken. Pentoses were easily utilized by the organism for growth and penicillin formation, when the media were properly prepared. The effectiveness of defatted chrysalis and defatted cotton seed as the source of nitrogen was compared with corn steep liquor, and it was proved that the defatted chrysalis can be used as the substitute of corn steep liquor. Good results were obtained, when defatted chrysalis was used in combination with corn steep liquor and especially when the pentose-media were previously refined by treating them with sodium aluminate and MgCl_2 to remove SiO_2. Thus, the growth and penicillin formation with pentose media could be attained to the level of those with lactose-corn steep liquor-media.Media and results are as follows : Basal constitutents : NaNO_3 0.3%, KH_2PO_4 0.25%, MgSO_4 0.05%, ZnSO_4 0.003% phenylacetylethanolamine 0.05%, CaCO_3 0.5% NaHSO_3 0.05%.Basal constituents plus(i)(Lactose 4% + corn steep liq. 4%.…890 u/c.c. (after 120 hrs.)Pentose 4.% + defatted chrysalis 3%.…826 u/c.c. (after 72 hrs)(ii)(Pentose (unrefined from rice hulls) 4%+corn steep Liq. 3%…662 u/c.c. (after 144 hrs)Pentose (refined, free from SiO_2) 4%+corn steep liq. 3%.…787 u/c.c. (after 96 hrs))
社団法人日本生物工学会の論文
1951-03-15