ハイブリドーマの培養液循環灌流培養法の開発

概要

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A perfusion culture was developed in which the cell-free culture supernatant was ultrafiltered, and the retentate was recycled to the culture vessel. One mouse-mouse hybridoma line and three mouse-human hybridoma lines were cultured by this method.In the culture of the mouse-mouse hybridoma 4C10B6 line using serum supplemented medium, the concentration of monoclonal antibody in the culture vessel was increased in proportion to culture time, but cell density decreased as time passed.In the cultures of 3 mouse-human hybridoma lines using serum-free medium, the concentration of monoclonal antibodies in culture vessels increased in proportion to culture time to 20 times that of a conventional perfusion culture, and viable cell densities were not less than those of conventional perfusion cultures.
公益社団法人日本生物工学会の論文
1988-01-25