パックブンの組織培養

概要

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Fundamental studies on the plant tissue culture of pak-bung, which we have used as a nutient absorber in advanced wastewater treatment, were conducted. The septum was proven to ge the best explant source for the tissue culture of pak-bung. Callus was easily formed from the septum portion on a modified MS medium which contained 1 mg/l of naphthaleneacetic acid (NAA) and benzyladenine (BA), and root and shoot redifferentiation were observed after 50 days of incubation. The capacity for callus formation and organ redifferentiation on two-times-diluted modified MS (1/2 MS) medium was better than that on MS medium. The media favored for callus formation were modified MS medium (NAA 1 mg/l, BA 1 mg/l) and 1/2 MS medium (NAA 1 mg/l, BA 1 mg/l), and the medium favored for organ redifferentiation was 1/2 MS medium (BA 0.05-1.0 mg/l). The specific growth rate of pak-bung callus on 1/2 MS agar medium (NAA 0.1 mg/l, 2,4-dichlorophenoxyacetic acid 1.0 mg/l, BA 0.5 mg/l) was 2.5 times greater than that on 1/2 MS medium (NAA 1 mg/l, BA 1 mg/l), but the callus formed was fragile and its water content was also high. Pak-bung callus was apt to change its colour to brown, but this browning could be retarded by the addition of ascorbic acid and citric acid.
社団法人日本生物工学会の論文
1987-03-25