わが国で見出されたジャガイモMウイルス

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A virus was isolated from apparently healthy plants of potato varieties, USDA seed1ing 41956 and Kintoki-imo. The virus was transmitted by sap, but not by aphids so far as the present study concerned. By sap inoculation, it produced ring-1ike 1ocal 1esions on the inoculated leaves of Nicotiana debneyi, while it caused systemic infection without symptoms in tomato and egg plant. Other plants such as Solanum demissum, S. villosum, Chenopodium amaranticolor, C. capitatum, C. murale, and Gomphrena globosa were also susceptible to the virus. Several potato varieties also became infected systemica11y. The virus was inactivatedin 10 minutes between 65 and 70℃, and between 2 and 3 days, at room temperature (ca. 20℃). Dilution end point was shown to 1ie between l:5,000 and l:10,000. In electron microscopy using direct negative staining method devised by Doi et al. (1965), e1ongated particles of about 650 mμ were observed. The virus was identified with potato virus M (PVM), because of its similarity to the description of PVM by Bagna11 et al. (1956) in host range, mode of transmission, symptomatology, physical properties, and particle morphology. Antisera against PVM and PVS (potato virus S) were prepared by intravenous injections of rabbits with partia11y purified virus preparations. The antiserum against PVM was shown to have a homologous titre of l:256 in precipitation test, and to be app1icable to side flocculation test. Result of cross precipitation tests between PVM-antiserum and PVS and vice versa, showed some serological relationship between PVM and PVS. In the potato varieties Norin No.1, 0ojiro, and Kennebec, mi1d to severe symptoms were observed on field-grown plants, natura11y infected with PVM. The characteristic symptoms associated with PVM were of veinal streak, glossy appearance of leaf surface, crink1ing, and downward Cur11ng of leaflets. Percentages of infection by PVM in seed potatoes maintained by the Tsumagoi National Potato Foundation Seed Farm were 11 % in Norin No. 1, 50 % in 0ojiro, 14 % in Kennebec, 31 % in Danshaku-imo, and almost 100 % in Saco, USDA Seed1ing 41956, and Kintoki-imo variety, respectively. With an exception of Norin NO. 1, these varieties were often symptomless carriers of PVM under field conditions.
日本植物病理学会の論文
1969-01-25

わが国で見出されたジャガイモMウイルス

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