土壌伝染性ムギモザイク病ウイルスの純化ならびに電子顕微鏡観察

概要

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Purification of two soil-borne viruses, namely wheat yellow mosaic virus and wheat green mosaic virus, was made. The morphology of these viruses was examined by the electron microscope. Purification procedure was as follows : juice from the diseased leaves was emulsified by adding one-fifth volume of chloroform. The emulsion was then centrifuged to give three layer separation. The top aqueous layer was found to contain the virus, the bottom chloroform layer contained the chlorophyll, and the middle compact plug formed by the chloroform gel contained protein and cellular debris. The virus was further purified by three cycles of differential centrifugation. The final preparation was infectious for wheat plants when rubbed on the leaves with carborundum. Electron microscopic examination of the purified preparation showed the presence of straight rod-shaped particles in both viruses. These particles were not found in a preparation from healthy plant leaves. The most frequent length of the virus particles was 120-180mμ (mode 150-160mμ) in the wheat yellow mosaic, and 120-180mμ (mode 170mμ) in the wheat green mosaic. The average width of both viruses was 25mμ. As shown by these results, the two viruses were indistinguishable in size and shape of particles.
日本植物病理学会の論文
1961-01-25