一環境要素として培養温度を考慮せる場合に於ける稻熱病菌の發育に及ぼす硫酸銅の影響に就て

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1. The relation of temperature to the growth of the strain of Pirlcularia Oryzae used in this experiment was first tested by growing the mycelium on potato decoction agar containing 1% sucrose at different temperatures. The most vigorous growth of mycelium seemed to occur at about 28℃. At the temperatures of about 24℃., 20℃., 11℃. and 36℃., the growth of the present fungus decreased gradually in order. 2. At about 28℃., the most favorable temperature to the fungus, the mycelial growth was stimulated by adding CuSo_4 to the agar medium in the concentrations between 1/400 and 1/6000 mol. The maximum growth occurred in the concentration of 1/1000 mol. In the case of the liquid medium the stimulating action of CuSo_4 to the mycelial growth of the fungus was more or less recognized. It was, however, not so remarkable as in the case of the agar modium. 3. At the various temperatures, such as 20℃., 24℃. and 32℃., the same expriments were done. However, the stimulating action of CuSo_4 to the mycelial growth was not recognized in any concentrations tested. At about 36℃. any cocelusive results were neither obtained in the liquid nor on the agar medium. 4. At all the temperatures tested, the conidial formation was retarded in inverse proportion to the amount of CuSo_4, When added in higher concentrations than 1/6000 mol. On the culture media containing CuSo_4 in higher concentrations than 1/400 mol., it was almost unrecognizable. 5. The conidial formation tends to decrease gradually with the difference of temperature from the optimum for the mycelial growth. Such retardation was most remarkable at about 36℃. 6. On the culture media containing CuSo_4 in higher concentrations than 1/600 mol., the chlamydospores were formed at all the temperatures tested. The limitation for the chlamydospore-formation with regard to the concentration of CuSo_4 tends to become lower in proportion to the difference of temparature from the optimum for the mycelial growth. Such tendency is quite contrary to that of the conidial formation. The devlopment of the aerial mycelium was checked gradually owing to the increment of the concentration of CuSo_4 and it was not observed in the concentration of 1/200 mol. at any temperatures tested. 8. On the culture media containing CuSo_4 in higher concentrion than 1/6000 mol., the color of the colony changed to gradually from grayish white to dark, and the degree of coloring increased remarkably in the concentration of 1/400-1/600 mol. In the concentration of 1/200 mol., the colony showed yellowish olive and soaked appearance. The hyphae became remarkably slenderer and more winding than those of the controls.
日本植物病理学会の論文
1930-03-31

一環境要素として培養温度を考慮せる場合に於ける稻熱病菌の發育に及ぼす硫酸銅の影響に就て

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