コンニャク葉枯病細菌の検出用選択培地

概要

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A selective medium (PPSM) was developed for the detection of Pseudomonas pseudoalcaligenes sub sp. konjaci (Ppk), the causal agent of bacterial leaf blight of konnyaku (Amorphophallus konjac), from plant debris and soil. The composition of the medium was as follows: 0.5 g KH_2PO_4, 3.0 g Na_2HPO_4・12H_2O, 6.0 g sodium tartrate, 2.0 g (NH_4) 2SO_4, 10μg L-leucine, 0.29 g MgSO_4・7H_2O, 67 mg CaCl_2・2H_2O, 0.25 mg Na_2M_0O_4・2H_2O, 20 mg phenol red, 2 mg methyl violet, 12.5 mg ampicillin sodium, 25 mg cycloheximide, 50 mg thiram-benomyl wettable powder, 15 g agar in 1 liter of distilled water. Colony forming efficiency of Ppk on PPSM was higher than that on King B medium. Reduction percent age of soil bacteria on PPSM ranged from 81.6 to 92.5% of the total number of soil bacteria recovered on nutrient broth yeast extract agar. Ppk was easily detected from the residue of diseased leaf let by using PPSM.
日本植物病理学会の論文
1987-10-25