ELISAによるイネ葉身中のイネいもち病菌の動態測定

概要

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We produced a polyclonal antibody which reacts with antigens of mycelia of the rice blast fungus. Using this antibody, we developed an ELISA method which can measure small amounts of mycelia of Magnaporthe grisea (Hebert) Barr in a rice leaf. More than 0.1 μg mycelia of the rice blast fungus in 100 mg of fresh leaf can be measured by this method. The antibody, acquired using lyophilized mycelia of the fungus, reacts as well with fresh mycelia, conidia, and even those of different races as it does with lyophilized ones. But it does not react with mycelia of several fungal pathogens of the rice plant. Thanatephorus cucumeris (Frank) Donk, Rhizoctonia sp., Cochliobolus miyabeanus (Ito et Kuribayashi) Drechsler ex Dastur, Sphaerulina oryzina Hara and Nigrospora oyzae (Berkeley et Broome) Petch do not react with the antibody at 20 μg dry weight mycelia/ml of PBS (pH 7.5) containing 0.05% Tween 80. But at about 2 mg/ml, the antibody slightly reacts with mycelia of Sphaerulina oyzina and Nigrospora oryzae. This method should be useful in understanding the interaction between blast fungus and rice plant.
日本植物病理学会の論文
1999-06-25