Enhanced Undecylprodigiosin Production from Serratia marcescens SS-1 by Medium Formulation and Amino-Acid Supplementation(BIOCHEMICAL ENGINEERING)

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Serratia marcescens Simon Swift-1 (SS-1) was used to produce a prodigiosin-like pigment, undecylprodigiosin (UP), known to have antitumor activities and potential as an anticancer drug. Modified media containing components of Luria-Bertani (LB) broth and selected amino acids were used to improve UP production from S. marcescens SS-1. Optimal culture conditions (e.g., temperature, pH, agitation rate) for UP production were also identified. It was found that S. marcescens SS-1 was able to produce 690mgl^<-1> of UP when it was grown with 5gl^<-1> yeast extract alone (YE medium) under the optimal culture conditions of 30℃, 200rpm, and pH8. The UP production of 690mgl^<-1> is nearly 23-fold of that obtained from original LB medium. Addition of amino acids containing pyrrole-like structures further enhanced UP production. Nearly 2 and 1.4gl^<-1> of UP was produced when the SS-1 strain was cultivated with YE medium supplemented with proline and histidine (5gl^<-1>), respectively. Moreover, the addition of aspartic acid (5gl^<-1>) also resulted in a high UP production of 1.4gl^<-1>. Optimal dosages of the three amino acids were subsequently determined and the highest UP production (2.5gl^<-1>) was achieved with the addition of 10gl^<-1> of proline. This suggests that the supplementation of amino acids related to the formation of a UP precursor (e.g., pyrrolylpyrromethene) could enhance UP production by the SS-1 strain.
社団法人日本生物工学会の論文
2005-10-25

Enhanced Undecylprodigiosin Production from Serratia marcescens SS-1 by Medium Formulation and Amino-Acid Supplementation(BIOCHEMICAL ENGINEERING)

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