Enhanced Production of Prodigiosin-like Pigment from Serratia marcescens SMΔR by Medium Improvement and Oil-Supplementation Strategies(Biochemical Engineering)
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概要
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Serratia marcescens SM△R, an SpnR-defective isogenic mutant of S. marcescens SS-1, was used to produce a prodigiosin-like pigment (PLP). Luria-Bertani (LB) broth, frequently used for prodigiosin biosynthesis with S. marcescens strains, was modified by increasing the concentrations of tryptone and yeast extract while completely removing NaCl from the medium. The resulting modified LB (MLB) medium achieved an almost 3.0-fold increase in PLP yield (152mg ℓ^<-1>) when compared with the original LB broth. The addition of vegetable oils (2-6% [v/v]) to the fermentation broth markedly enhanced PLP production. PLP yields of 525, 579, and 790mg ℓ^<-1> were obtained when the MLB medium was supplemented with 4% soybean oil, 4% olive oil and 6% sunflower oil, respectively. PLP production was found to be positively correlated with extracellular surface emulsification activity, suggesting a link between the PLP production and the presence of biosurfactant. This work shows that the optimal medium for PLP yield was sunflower oil (6%)-supplemented MLB medium, which resulted in an approximately 14-fold higher PLP yield than that in LB broth. Mass spectrometry and NMR analysis indicated that the PLP product is a prodigiosin derivative, called undecylprodigiosin.
- 2005-06-25
著者
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Chen Wei-chuan
Graduate School Of Biotechnology And Bioinformatics Yuan-ze University
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Chen Wei
Graduate School Of Biotechnology And Bioinformatics Yuan-ze University
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Wei Yu-hong
Graduate School Of Biotechnology And Bioinformatics Yuan-ze University
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Wei Yu
Graduate School of Biotechnology and Bioinformatics, Yuan-Ze University
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Chen Wei-chuan
Graduate School Of Biotechnology And Bioengineering Yuan Ze University
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Wei Yu
Graduate School Of Biotechnology And Bioinformatics Yuan-ze University
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Wei Yu-hong
Graduate Institute Of Biotechnology And Bioinformatics Yuan-ze University
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