Purification of Turkey Pancreatic Phospholipase A_2(Biochemistry & Molecular Biology)

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概要

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• Turkey pancreatic phospholipase (TPP) has been purified from delipidated pancreases. The purification included ammonium sulfate fractionation, acidic (pH 5) treatment, followed by sequencial column chromatographies on DEAE-cellulose, Sephadex G-75, and reverse phase high pressure liquid chromatography. The purified enzyme was found to be a monomeric protein with molecular mass of 14kDa. The optimal activity was measured at pH 8 and 37℃ using egg yolk emulsion as substrate. Our results show that the enzyme (TPP) was not stable for 1 h at 60℃, and that bile salt and Ca^<2+> were required for the expression of the purified enzyme. The sequence of the N-terminal amino acids of the purified enzyme shows a very close similarity between TPP and all other known pancreatic phospholipases.

• 社団法人日本農芸化学会の論文
• 2003-10-23

著者

• Salah Riadh

  Laboratoire De Biochimie Et De Genie Enzymatique Des Upases Ecole Nationale Des Ingenieurs De Sfax

• Salah Riadh

  Laboratory Of Biochemistry

• Mejdoub Hafedh

  Laboratoire de Biochimie et de Genie enzymatique des Upases, Ecole Nationale des Ingenieurs de Sfax

• Mejdoub Hafedh

  Laboratoire De Biochimie Et De Genie Enzymatique Des Lipases

• Mejdoub Hafedh

  Laboratory Of Biochemistry Faculty Of Sciences Of Sfax

• ZOUARI Nacim

  Laboratory of Biochemistry

• REINBOLT Joseph

  IBMC

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• Purification of Turkey Pancreatic Phospholipase A_2(Biochemistry & Molecular Biology)
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