Immobilization of Leukemia Inhibitory Factor (LIF) to Culture Murine Embryonic Stem Cells(CELL AND TISSUE ENGINEERING)

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概要

• 論文の詳細を見る

• Murine embryonic stem (ES) cells were cultured on a material containing immobilized leukemia inhibitory factor (LIF). To immobilize LIF, we synthesized photoreactive gelatin mixed with LIF and cast the mixture on a polystyrene plate, which was then dried. LIF was immobilized by photo-irradiation in the presence or absence of a photo mask. The plate was washed until LIF was no longer released. Murine ES cells were cultured on the immobilized LIF. Activation of STAT3 was maintained on the immobilized LIF for 6 d even after removing soluble LIF. Oct-3/4 was also expressed in the cells cultured on the immobilized LIF. As a result, the mouse ES cells were cultured without differentiating on the immobilized LIF for 6 d. It was possible to culture murine ES cells without adding soluble LIF at each medium change. We conclude that our material containing immobilized LIF might be useful in the culture of murine ES cells.

• 公益社団法人日本生物工学会の論文
• 2004-11-25

著者

• ITO Yoshihiro

  Kanagawa Academy of Science and Technology

• Hasuda Hirokazu

  Kanagawa Academy of Science and Technology

• Hasuda Hirokazu

  Kanagawa Acad. Of Sci. And Technol.

• MAKINO HIROSHI

  Kanagawa Academy of Science and Technology

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• Immobilization of Leukemia Inhibitory Factor (LIF) to Culture Murine Embryonic Stem Cells(CELL AND TISSUE ENGINEERING)

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