A Long Acidic Domain Affects the Chromatographic Behaviour of a Neuronal Adaptor Protein on DEAE-Sepharose(Biochemistry & Molecular Biology)

スポンサーリンク

概要

• 論文の詳細を見る

• The stepwise chromatographic behaviour on DEAE-Sepharose of rat Fe65, a neuronal protein, was tested, using as eluants KCl, CaCl_2, and MgCl_2. Assays by western blot showed that Fe65 was eluted by CaCl_2, at a ionic strength 20% lower than that of MgCl_2 or KCl. Interestingly, in the case of a truncated Fe65, lacking a glutamic acid rich region at the N-terminus, the ionic strengths of the various eluants were almost identical. These results suggested a possible inhibitory role of calcium ions in the binding of the protein to DEAE and a specific affinity of these ions for long acidic stretches.

• 社団法人日本農芸化学会の論文
• 2003-09-23

著者

• Zambrano Nicola

  Dipartimento Di Biochimica E Biotecnologie Mediche Universita Di Napoli Federico Ii

• Longo Olimpia

  Dipartimento Di Biochimica E Biotecnologie Mediche Universita Di Napoli Federico Ii

• Arcari Paolo

  Dipartimento Di Biochimica E Biotecnologie Mediche Universita Di Napoli Federico Ii

• Lamberti Annalisa

  Dipartimento Di Biochimica E Biotecnologie Mediche Universita Di Napoli Federico Ii

• LONGO Olimpia

  Department of Biochemistry and Medical Biotechnologies, University of Naples Federico II

• LAMBERTI Annalisa

  Department of Biochemistry and Medical Biotechnologies, University of Naples Federico II

• ZAMBRANO Nicola

  Department of Biochemistry and Medical Biotechnologies, University of Naples Federico II

• ARCARI Paolo

  Department of Biochemistry and Medical Biotechnologies, University of Naples Federico II

関連論文

• Probing the Secondary Structure of a Recombinant Neuronal Adaptor Protein and Its Phosphotyrosine Binding Domains
• A Long Acidic Domain Affects the Chromatographic Behaviour of a Neuronal Adaptor Protein on DEAE-Sepharose(Biochemistry & Molecular Biology)

スポンサーリンク