A Root-specific 0-Methyltransferase Gene Expressed in Salt-tolerant Barley(Biochemistry & Molecular Biology)

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概要

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• A cDNA encoding an O-methyltransferase (OMT) was isolated from salt-tolerant barley roots by subtraction hybridization with cDNAs of salt-tolerant barley roots as a tester cDNA and cDNAs of the salt-sensitive barley roots as a driver cDNA. The deduced amino acid sequence showed significant identity with plant caffeic acid/5-hydroxyferulic acid OMTs. Southern blot analysis showed that the OMT gene was a single copy in both salt-tolerant and -sensitive barley. The cloned gene was expressed in a wheat germ cell-free system to produce the OMT, which had methylating activity for caffeic acid. Northern blot analysis showed that the OMT gene was expressed constitutively in the salt-tolerant barley roots and the expression level was increased 1.5 times by salt stress, but the salt-sensitive barley showed no expression of the gene in roots and leaves.

• 社団法人日本農芸化学会の論文
• 2003-05-23

著者

• Sugimoto Manabu

  Laboratory Of Biochemistry Research Institute For Bioresources Okayama University

• Ito Kazutoshi

  Plant Bioeng.res.lab.sapporo Brew.ltd.

• Sato Kazuhiro

  Laboratory Of Biochemistry Research Institute For Bioresources Okayama University

• Okada Yoshihiro

  Plant Bioengineering Research Laboratories Sapporo Breweries Ltd.

• TAKEDA Kazuyoshi

  Laboratory of Biochemistry, Research Institute for Bioresources, Okayama University

• Takeda Kazuyoshi

  Laboratory Of Biochemistry Research Institute For Bioresources Okayama University

関連論文

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• A Root-specific 0-Methyltransferase Gene Expressed in Salt-tolerant Barley(Biochemistry & Molecular Biology)
• Putative phospholipid hydroperoxide glutathione peroxidase gene from Arabidopsis thaliana induced by oxidative stress
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