Biosynthesis of Rice Phytoalexin : Enzymatic Conversion of 3β-Hydroxy-9β-pimara-7,15-dien-19,6β-olide to Momilactone A(Organic Chemistry)
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概要
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Momilactone A, a major rice diterpene phytoalexin, could be synthesized by dehydrogenation at the 3-position of 3β-hydroxy-9β-pimara-7,15-dien-19,6β-olide in rice leaves. The presence of 3β-hydroxy-9β-pimara-7,15-dien-19,6β-olide in UV-irradiated rice leaves was confirmed by comparing the mass spectra and retention times after a GC/MS analysis of the natural and synthetic compounds. The soluble protein fraction from UV-irradiated rice leaves showed dehydrogenase activity to convert 3β-hydroxy-9β-pimara-7,15-dien-19,6β-olide into momilactone A. The enzyme required NAD^+ or NADP^+ as a hydrogen acceptor. The optimum pH for the reaction was 8. The K_m value to 3β-hydroxy-9β-pimara-7,15-dien-19,6β-olide was 36 μM when NAD^+ was supplied as a cofactor at a concentration of 1 mM. 3β-Hydroxy-9β-pimara-7,15-dien-19,6β-olide and its dehydrogenase activity were induced in a time-dependent manner by UV irradiation.
- 社団法人日本農芸化学会の論文
- 2002-03-23
著者
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Kodama Osamu
United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology:labora
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Hasegawa Morifumi
United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology:labora
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Hasegawa Morifumi
United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology
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ATAWONG Anotai
United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology
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Atawong Anotai
United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology
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Kodama Osamu
United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology
関連論文
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- Biosynthesis of Rice Phytoalexin : Enzymatic Conversion of 3β-Hydroxy-9β-pimara-7,15-dien-19,6β-olide to Momilactone A(Organic Chemistry)
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