Use of Shark Collagen for Cell Culture and Zymography(Biochemistry & Molecular Biology)

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概要

• 論文の詳細を見る

• The uses of shark collagen as a matrix for cell culture and as a substrate for zymography were investigated. Fibroblasts were cultured on a gel matrix of shark type I collagen at 30℃. The collagen gel had contracted by 4 days of incubation. Individual fibroblasts were visible against the transparent background of the contracted collagen as long, lean star-shaped cells. The matrix metalloproteinases (MMPs) from fibroblasts secreted from the medium more easily digested shark gelatin than pig gelatin. MMP-2, -9, and that of potential form were recognizable in the zymographic gel of shark gelatin.

• 社団法人日本農芸化学会の論文
• 2002-12-23

著者

• Nomura Yoshihiro

  Department Of Applied Protein Chemistry Faculty Of Agriculture Tokyo University Of Agriculture And T

• Nomura Yoshihiro

  Department Of Applied Protein Chemistry Faculty Of Agriculture Tokyo University Of Agriculture And T

• KITAZUME Nobuhide

  Department of Applied Protein Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and

• Kitazume Nobuhide

  Department Of Applied Protein Chemistry Faculty Of Agriculture Tokyo University Of Agriculture And T

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