Molecular Characterization of Sucrose : Sucrose 1-Fructosyltransferase and Sucrose : Fructan 6-Fructosyltransferase Associated with Fructan Accumulation in Winter Wheat during Cold Hardening(Biochemistry & Molecular Biology)

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概要

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• We isolated two cDNAs of winter wheat (Triticum aestivum L.), designated wft1 and wft2, which encoded sucrose : fructan 6-fructosyltransferase (6-SFT) and sucrose : sucrose 1-fructosyltransferase (1-SST ; EC 2. 4. 1. 99), respectively, which are involved in the synthesis of fructan in wheat. wft1 and wft2 were cloned by screening of a cDNA library with probed-cDNA fragments corresponding to plant fructosyltransferase and invertase. The identity of the clones was verified by functional characterization of recombinant proteins expressed in methylotrophic yeast, Pichia pastoris. Northern blotting showed that the level of wft2 transcripts increased from autumn to early winter in the crown tissues of all field-grown wheat cultivars examined. Higher levels of wftl and wft2 transcripts were found in leaf tissues of snow mold-resistant cultivars, which accumulated more fructan than other cultivars. Our results showed that Wft1 and Wft2 were important in fructan accumulation during cold hardening of winter wheat.

• 社団法人日本農芸化学会の論文
• 2002-11-23

著者

• Yoshida Midori

  National Agricultural Research Center For Hokkaido Region

• Kawakami Akira

  National Agricultural Research Center for Hokkaido Region

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