Isolation and Characterization of Basic Exochitinase from Leaf Extract of Rehmannia glutinosa

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概要

• 論文の詳細を見る

• Rehmannia chitinases were extracted from the leaves of Rehmannia glutinosa under acidic conditions (pH 2.9). We purified a 28.6-kDa chitinase, designated as P2,from crude extract to homogeneity by (NH_4)_2SO_4 precipitation, chromatography with regenerated chitin affinity and hydrophobic interaction column, and preparative native PAGE. Isolated P2 showed maximum chitinase activity at pH 5.0 and 60℃, and had a isoelectric point of 8.46. P2 produced only (GlcNAc)_2 from (GlcNAc)_4-6 and regenerated chitin. Based on these results, we arrived at the conclusion that P2 was a basic exochitinase.

• 社団法人日本農芸化学会の論文
• 1999-10-23

著者

• Pan C‐h

  Seoul National Univ. Suwon Kor

• Kim S‐i

  Seoul National Univ. Suwon Kor

• Pan Cheol-ho

  Department Of Agricultural Chemistry And Research Center For New Bio-materials In Agriculture Colleg

• Lee Euna

  Department Of Agricultural Chemistry And Research Center For New Bio-materials In Agriculture Colleg

• PAN CheolHo

  Department of Agricultural Chemistry and Research Center for New Bio-Materials in Agriculture, Colle

• SON JongMun

  Department of Agricultural Chemistry and Research Center for New Bio-Materials in Agriculture, Colle

• KIM SuIl

  Department of Agricultural Chemistry and Research Center for New Bio-Materials in Agriculture, Colle

• Son Jongmun

  Department Of Agricultural Chemistry And Research Center For New Bio-materials In Agriculture Colleg

• Lee Eun-A

  Department of Agricultural Chemistry and Research Center for New Bio-Materials in Agriculture, College of Agriculture and Life Sciences, Seoul National University

• Son Jong-Mun

  Department of Agricultural Chemistry and Research Center for New Bio-Materials in Agriculture, College of Agriculture and Life Sciences, Seoul National University

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• Isolation and Characterization of Basic Exochitinase from Leaf Extract of Rehmannia glutinosa
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