Purification of Acetyl Coenzyme A : Deacetylacephalosporin C O-Acetyltransferase from Acremonium chrysogenum

概要

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Acetyl CoA : deacetylcephalosporin C O-acetyltransferase, which catalyzes the final step of the biosythetic pathway to cephalosporin C, was stabilized by a buffer solution containing 7-aminocephalosporanic acid and purified over 1300-fold from Acremonium chrysogenum. The purified enzyme has a molecular weight of 55,000 as measured by gel filtrantion. SDS-polyacrylamide gel electrophoresis showed two subunit bands corresponding to molecular weights of 27,000 and 14,000. The enzyme has an isoelectoric point at pH 4.0 and optimum activity at pH 7.5.
社団法人日本農芸化学会の論文
1992-09-23