Optimal Conditions for Frozen Storage of Immobilized Luminous Bacteria

概要

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With a view to utilizing a luminous material, a cake containing immobiliezed luminous bacteria, for attracting fish, the frozen storage of the luminous material was studied.Glycerol, sorbitol, and DL-threonine had some protecting effect against the damage to luminous bacteria during freezing and thawing, and strong light intensity of the luminous material was obtained at 4 hr after thawing with the addition of one of these substances to the culture broth before immobilization. Among several strains of Photobacterium phosphaoreum, strain MT-10208 was the most resistant against the damage caused by freezing and thawing, and the luminous material consisting of cells of the same strain immobilized in calcium alginate gel showed the strongest light intensity after thawing. Gradual freezing at -15℃ was preferable to maintain the light intensity of luminous material, but rapid freezing at temperatures lower than -50℃ was harmful to luminous activity. The time required for recovering the light intensity after thawing differed depending on the period of storage and the addition of a protecting substance, and the luminous material was stored successfully without losing its bioluminescence for at least 32 days by carefully selecting the freezing and thawing conditions.
公益社団法人日本生物工学会の論文
1980-08-25