Constitutive Trichloroethylene Degradation Led by tac Promoter Chromosomally Integrated Upstream of Phenol Hydroxylase Genes of Ralstonia sp. KN1 and Its Nucleotide Sequence Analysis

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概要

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• Ralstonia sp. KN1-10A is a strain capable of degrading trichloroethylene (TCE) constitutively due to the tac promoter (Ptac) integrated upstream of the phenol hydroxylase genes (phy) in its chromosome. The expression of Ptac was analyzed using luxAB of Vibrio harveyi as a reporter. After determining the nucleotide sequence of phyABCDE required for TCE degradation, a luxAB-encoding fragment was integrated downstream of phyE by homologous recombination in strain KN1-10A, obtaining strain KN1-10A-LX. In the same manner, the luxAB-encoding fragment was integrated into the chromosome of the wild-type strain, KN1. The resultant strain KN1-LX was used to analyze the gene expression caused by phenol induction. The expression induced by Ptac was compared to that by phenol induction. Although the level of luxAB expression led by Ptac was almost equal to that induced by phenol, the TCE degradation rate by the Ptac-carrying KN1-10A-LX was markedly slower than that by the phenol-induced KN1-LX. These results suggesest that an important gene for TCE degradation was not transcribed by Ptac in KN1-10A-LX. The nucleotide sequence analysis showed the existence of a small gene, phyZ, upstream of phyA, and Ptac was found to be integrated into the middle of phyZ in KN1-10A-LX. The effect of phyZ on TCE degradation was examined by using recombinant strains expressing phyABCDE with or without phyZ in a plasmid. The coexistence of phyZ markedly accelerated TCE degradation. Through an exhaustive expression analysis, it was demonstrated that the chromosomal integration of Ptac was a very attractive method for high and stable production of phenol hydroxylase for TCE degradation.

• 社団法人日本生物工学会の論文
• 2000-01-25

著者

• Nakamura Kanji

  Corporate Research And Development Center Kurita Water Industries Ltd.

• ISHIDA Hiroaki

  Corporate Research & Development Laboratories, Sumitomo Metal Industries, Ltd.

• IIZUMI TARO

  Corporate Research & Developmen t Center, Kurita Water Industries Ltd.,

• Iizumi Taro

  Corporate Research And Development Center Kurita Water Industries Ltd.

• Nakamura Kanji

  Corporate Research & Developmen T Center Kurita Water Industries Ltd.

• Ishida Hiroaki

  Corporate Research And Development Center Kurita Water Industries Ltd.

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• Constitutive Trichloroethylene Degradation Led by tac Promoter Chromosomally Integrated Upstream of Phenol Hydroxylase Genes of Ralstonia sp. KN1 and Its Nucleotide Sequence Analysis

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