Breeding of High-Pyruvate-Producing Torulopsis glabrata with Acquired Reduced Pyruvate Decarboxylase

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概要

• 論文の詳細を見る

• An efficient method for isolation of pyruvate-producing mutants of Torulopsis glabrata IFO 0005 was established. On mutagenesis of the parent strain, mutants requiring acetate for complete growth were isolated. The acetate-leaky auxotrophic mutants demonstrated higher fermentative yields of pyruvate from glucose than the parent strain. Thus, the pyruvate decarboxylase(PDC)activity of mutants requiring acetate for complete growth was lower than that of the parent strain. A decrease in the PDC activity would therefore block the formation of acetate via acetaldehyde and thus increase pyruvate production. Among the mutants, T.glabrata ACII-33 had accumulated free pyruvic acid(60.3 g/l)in 47h(yield, 67.3% ; conversion of pyruvic acid from glucose consumed)in a 3l jar-fermentor. This yield by strain ACII-33 represented a 15% increase compared to that with the parent strain.

• 公益社団法人日本生物工学会の論文
• 1999-08-25

著者

• Yonehara Tetsu

  R&d Planning Department Toray Industries Inc.

• MIYATA REIKO

  Chemicals Research Laboratories, Toray Industries Inc.

• YONEHARA TETSU

  R&D Planning Department, Toray Industries Inc.

• Miyata Reiko

  Chemicals Research Laboratories Toray Industries Inc.

関連論文

• Breeding of High-Pyruvate-Producing Torulopsis glabrata with Acquired Reduced Pyruvate Decarboxylase
• Breeding of High-Pyruvate-Producing Torulopsis glabrata and Amino Acid Auxotrophic Mutants
• Improvement of Fermentative Production of Pyruvate from Glucose by Torulopsis Glabrata IFO 0005 : Studies on Pyruvate Production by Yeast (II)
• Fermentative Production of Pyruvate from Glucose by Torulopsis glabrata : Studies on Pyruvate Production by Yeast (I)

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