Fermentative Production of Pyruvate from Glucose by Torulopsis glabrata : Studies on Pyruvate Production by Yeast (I)

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Novel strains selected from various genera of yeasts were investigated with regard to pyruvate productivity from glucose. The ability to produce pyruvate was found in yeasts belonging to the genera Saccharomyces, Toluropsis, Pichia, Candida and Trichosporon with a new resting cell method using the thiamine analogue, oxythitamine. In this first screening, strains belonging to the genus Saccharomyces showed the highst pyruvate productivity. In secondary screeing using a fermentative method, strains belonging to the genus Torulopsis showed higher pyruvate productivity, with Torulopsis glabrate exhibiting the highest fermentative pyruvate productivity. These pyruvate producing yeast strains were divided into two types. The first type of yeast showed a high accumulation of pyruvate with the resting cell method, but a low accumulation with the fermentative method. The second type of yeast showed a high accumulation of pyruvate with the fermentative method, but a low accumulation with the resting cell method. The first type did not commonly possess thiamine-auxotrophy, while the second type exhibited at least double auxotrophy fot both biotin and thiamine. All six strains belonging to T. glabrata required four vitamins; nicotinic acid, thiamine, pyridoxine and biotin for their growth and accumulated a high conversion of pyruvate (53.7mM-300mM)under the fermentative screening conditions. T. glabrate IFO 0005 was selected as the best strain among the T. glabrata. The optimum concentrations of these vitamins for the highest fermentative pyruvate productivity by T. glabrata IFO 0005 were determined. Thiamine and nicotinate were more effective towards productivity than other vitamins. The accumulation of pyruvic acid reached a final concentration of 648mM (57.0g/l as free pyruvic acid)at 59 h from an initial glucose concentration of 100g/l by T. glabrata IFO 0005 nuder the optimal vitamin concentrations determined in this study. Consequently, the auxotrophy of multiple vitamins which are involved in the metabolism of pyruvate proved to be highly effective in the fermentative production of pyruvate from glucose.
公益社団法人日本生物工学会の論文
1994-08-25

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