Purification and Characterization of a Bacteriocin Produced by Lactococcus lactis subsp.lactis H-559 Isolated from Kimchi

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概要

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• Lactic acid bacteria were isolated from kimchi and screened for bacteriocin production. Strain H-559, identified as Lactococcus lactis subsp.lactis, exhibited the strongest antibacterial activity among them and was active against pathogenic bacteria such as Listeria monocytogenes and Staphylococcus aureus as well as many lactic acid bacteria. The antimicrobial substance produced by L.lactis subsp.lactis H-559 was inactivated by α-chmotrypsin, and protease type IX and XIV and was confirmed to be a bacteriocin. The bacteriocin activity was stable from pH 2.0-11.0 and up to 10 min heating at 100℃. The bacteriocin was sequentially purified by ammonium sulfate precipitation, ion-exchange chromatography, and reversed-phase high-performance liquid chromatography(HPLC). Its molecular weight was determined to be 3343.7 Da by MALDI-mass spectrometry. Isoleucine was detected as the first N-terminal amino acid residue but the remaining amino acid sequence could not be determined by the Edman degradation method. It was different from other bacteriocins in terms of pH stability, molecular weight, amino acid composition, and the partial amino acid sequences of peptides obtained by acid hydrolysis.

• 社団法人日本生物工学会の論文
• 1999-08-25

著者

• Ahn Jong-seog

  Cellular Response Modifier Research Unit Korea Research Institute Of Bioscience And Biotechnology

• Hwang In-kyeong

  Department Of Food Science And Nutrition Seoul National University

• Kim S‐h

  Protein Function Research Unit Korea Research Institute Of Bioscience And Biotechnology

• KIM Seung-Ho

  Protein Function Research Unit, Korea Research Institute of Bioscience and Biotechnology

• Mheen Tae-ick

  Cellular Response Modifier Research Unit Korea Research Institute Of Bioscience And Biotechnology

• Joo Yun-jung

  Cellular Response Modifier Research Unit Korea Research Institute Of Bioscience And Biotechnology

• Lee Hun-joo

  Cellular Response Modifier Research Unit Korea Research Institute Of Bioscience And Biotechnology

• Kim Seung-ho

  Protein Function Research Unit Korea Research Institute Of Bioscience And Biotechnology

• Park Chan-sun

  Cellular Response Modifier Research Unit Korea Research Institute Of Bioscience And Biotechnology

• Kim Seung-Ho

  Protein Engineering Group, Korea Research Institute of Bioscience and Biotechnology

関連論文

• Structures of Ovine Corticotropin-Releasing Factor and Its Ala32 Mutant as Studied by CD and NMR Techniques
• Inhibition of Telomerase Activity by Fungus Metabolites, CRM646-A and Thielavin B
• Purification and Characterization of a Bacteriocin Produced by Lactococcus lactis subsp.lactis H-559 Isolated from Kimchi
• Purification and Characterization of Subtilisin DJ-4 Secreted by Bacillus sp. Strain DJ-4 Screened from Doen-Jang
• Structures of Ovine Corticotropin-Releasing Factor and Its Ala32 Mutant as Studied by CD and NMR Techniques.

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