Utilization of Swine Wastewater as a Feedstock for the Production of Polyhydroxyalkanoates by Azotobacter vinelandii UWD

概要

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Azotobacter vinelandii UWD produced 0.69 g・l^<-1> poly(hydroxybutyrate-co-hydroxyvalerate, PHBV) with 7.9 mol% hydroxyvalerate (HV) from twofold-diluted swine wastewater (SW). When supplemented with 20 g・l^<-1> glucose, twofold-diluted SW increased copolymer production by 8.6 times. When three organic acids (acetate, propionate and butyrate) present in SW were supplemented with 20 g・l^<-1> glucose, PHBV production was comparable (5.5 g・l^<-1>) to that in the case of using twofold-diluted SW supplemented with 20 g・l^<-1> glucose. However, the HV level (1.1-1.3 mol%) was very low. The component in SW contributing to copolymer production was found to be valerate. By 20 mM valerate 0.2 g・l^<-1> PHBV with 44.6 mol% HV was produced. With 30 g・l^<-1> glucose supplementation, 4.0 g・l^<-1> PHBV with 22 mol% HV was produced. The optimal ratios of carbon to phosphorus (C : P) and to nitrogen (C : N) were 165:1 and 22:1, respectively.
社団法人日本生物工学会の論文
2001-02-25