Changes in Serological Reactivity of Papaya leaf distortion mosaic virus Caused by Papain in Carica papaya L. and Its Detection Using Antipain or Papain
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概要
- 論文の詳細を見る
Losses in serological reactivity of Papaya leaf distortion mosaic virus (PLDMV) were demonstrated. An antibody, IgG-papaya, raised against PLDMV purified from papaya (Carica papaya L.) did not react with virus particles in Cucumis metuliferus leaf extracts in ELISA or SSEM-PAG (serologically specific electron microscopy using protein A-gold). In addition, IgG-papaya and IgG-Cucumis raised against PLDMV purified from C. metuliferus did not react with virus particles in papaya leaf extracts after western blotting. From results of electrophoresis, the coat protein (CP) of PLDMV purified from papaya had degraded and migrated in two bands. Similar degradation was also observed when virus purified from C. metuliferus was treated with papain. These results indicated that the CP of PLDMV purified from papaya was degraded during the purification process by papain in the host plant. IgG-papaya was reactive to papaindegraded CP, while IgG-Cucumis was reactive to both intact and degraded CP. Modified serological methods using antipain (a protease inhibitor) or papain were established to detect PLDMV.
- 2002-02-25
著者
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Tsuda Shinya
National Agriculture Research Center
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Tsuda Shinya
National Agricultural Research Center
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Usugi Tomio
National Agriculture Research Center
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MAOKA Tetsuo
National Agricultural Research Center for Hokkaido Region
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NODA Chiyoichi
Okinawa Subtropical Station of the Japan International Research Center for Agricultural Sciences
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IWASAKI Mabito
National Agricultural Research Center for Hokkaido Region
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Usugi Tomio
National Agricultural Res. Center Tsukuba Jpn
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Tsuda Shinya
National Agricultural Res. Center Ibaraki Jpn
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Usugi Tomio
National Agricultural Res. Center Ibaraki Jpn
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