Similarity between Copper Resistance Genes from Pseudomonas syringae pv. actinidiae and P. syringae pv. tomato

スポンサーリンク

概要

• 論文の詳細を見る

• Twenty-eight strains of Pseudomonas syringae pv. actinidiae isolated in 1984, 1987 and 1988 from kiwifruit orchards in Japan were tested for their resistance to copper sulfate. All strains isolated in 1984 were copper sensitive with a minimum inhibitory concentration (MIC) of cupric sulfate of 0.75 mM. However, some strains isolated in 1987 and 1988 were resistant, with the MIC ranging from 2.25 to 3.0 mM. All copper-resistant strains contained at least one of two plasmids, pPaCul (about 70.5 kb) or pPaCu2 (about 280 kb), or both. In a copper-resistant strain Pa429, the location of the copper-resistance gene(s) was examined by insertional inactivation with Tn5. The MIC of copper sulfate in the copper-sensitive mutant obtained by Tn5 tagging decreased from 2.75 to 0.75 mM. The 14.5 kb BamHI fragment, designated pPaCuB14, containing the same locus mutagenized with Tn5 was cloned from pPaCu1. However, pPaCuB14 did not confer copper resistance in the transformant of copper-sensitive strain Pa21R, suggesting that this clone did not contain a full set of copper-resistance gene(s). Then a cosmid library of pPaCu1 was constructed and six cosmid clones hybridized with pPaCuB14 were selected. One of the six cosmids, designated pPaCuC1, conferred a near wild-type level of copper resistance in the transformant of the copper-sensitive strain. pPaCuC1 had a homologous region that hybridized with all of the PCR-amplified fragments of copA, copB, copR, and copS genes of P. syringae pv. tomato. DNA sequence analysis of the homologous region revealed the existence of four open reading frames (ORF A, B, R and S) oriented in the same direction. The predicted amino acid sequences of ORF A, B, R and S had 80, 70, 97 and 95% identity with CopA, B, R and S of P. syringae pv. tomato, respectively.

• 日本植物病理学会の論文
• 2002-02-25

著者

• NAKAJIMA Masami

  Department of Ophthalmology, School of Medicine, Nihon University

• HIBI Tadaaki

  Department of Agricultural and Environmental Biology, The University of Tokyo

• GOTO Masao

  Faculty of Agriculture, Shizuoka University

• Nakajima Masami

  Department Of Agricultural And Environmental Biology The University Of Tokyo:(present Address)school

• Hibi Tadaaki

  Department Of Agricultural And Environmental Biology The University Of Tokyo

• Goto Masao

  Faculty Of Agriculture Shizuoka University

関連論文

• Twenty-gauge Transconjunctival Vitrectomy
• Correlation Between Indocyanine Green Angiographic Findings and Histopathology of Polypoidal Choroidal Vasculopathy
• Thiadiazole carboxylic acid moiety of tiadinil, SV-03, induces systemic acquired resistance in tobacco without salicylic acid accumulation
• Aster yellows subgroup (Candidatus Phytoplasma sp. AY 16S-group, AY-sg) phytoplasma associated with porcelain vine showing witches' broom symptoms in South Korea
• Characterization of a phytoplasma associated with witches' broom disease of potatoes in Korea
• Transgenic cucumber expressing an endogenous class III chitinase gene has reduced symptoms from Botrytis cinerea
• Response of transgenic cucumber expressing a rice class I chitinase gene to two fungal pathogens with different infectivities
• Influence of bacteria isolated from rice plants and rhizospheres on antibiotic production by the antagonistic bacterium Serratia marcescens strain B2
• Biological control of rice blast by the epiphytic bacterium Erwinia ananas transformed with a chitinolytic enzyme gene from an antagonistic bacterium, Serratia marcescens strain B2
• Synergistic Antifungal Activity of Chitinolytic Enzymes and Prodigiosin Produced by Biocontrol Bacterium, Serratia marcescens Strain B2 against Gray Mold Pathogen, Botrytis cinerea
• Functional Analysis of an ATP-Binding Cassette Transporter Gene in Botrytis cinerea by Gene Disruption
• Cloning and Selective Toxicant-induced Expression of BMR1 and BMR3,Novel ABC Transporter Genes in Botrytis cinerea
• Indocyanine Green Angiography and Histopathology of Choroidal Neovascular Membrane in Age-Related Macular Degeneration
• Simple and Sensitive Method for the Detection of Escherichia coli in Natural Environments Using the Ice Nucleation Active Gene
• Similarity between Copper Resistance Genes from Pseudomonas syringae pv. actinidiae and P. syringae pv. tomato
• Distribution of the Consensus Sequences of ABC Transporter Gene among Several Taxonomically Distinct Phytopathogenic Fungi (Short Communication)
• Evaluation of sleep using ambulatory skin potential recording : Differences between morning and evening type
• Correlation Between Angiographic and Pathological Findings in Experimental Choroidal Neovascular Membranes After Transpupillary Thermotherapy
• Ethylene Production by the Kudzu Strains of Pseudomonas syringae pv. phaseolicola Causing Halo Blight in Pueraria lobata (Willd) Ohwi
• Ethylene Production by Cell-Free Extract of the Kudzu Strain of Pseudomonas syringae pv. Phaseolicola
• Metabolism of Collagen and Muscle Protein of Adult Rats in Protein Depletion and Repletion

もっと見る 閉じる

スポンサーリンク