Purification and Partial Characterization of Figaren, an RNase-like Novel Antiviral Protein from Cucumis figarei

スポンサーリンク

概要

• 論文の詳細を見る

• An antiviral protein, designated figaren, was purified from leaves of Cucumis figarei and partially characterized. Column chromatography, SDS-polyacrylamide gel electrophoresis and periodic acid-Schiff staining revealed that rigaten is a glycoprotein with a molecular weight of 23 kDa. Figaren was stable at pH 2 to 12 and below 90℃. N-terminal amino acid sequencing indicated that figaren contained the conserved region for the S-allele-associated ribonucleases (RNases). In-gel RNase assay showed that rigaten digested yeast RNAs. Figaren also digested double-stranded RNAs extracted from Cucumber mosaic virus (CMV)-infected tobacco tissues. Fluorescence in situ hybridization revealed that figaren and RNases (beef pancrease and RNase T_1) at μg/ml similarly inhibited CMV infection in cowpea leaves. Figaren and the RNases at 5-500 ng/ml had similar inhibitory effect on local lesion formation by CMV. These data suggest that figaren is a novel RNase-like antiviral protein. (Received October 10, 2000 ; Accepted February 6, 2001)

• 日本植物病理学会の論文
• 2001-05-25

著者

• Ohki T.satoshi

  Graduate School Of Agriculture And Biological Sciences Osaka Prefecture University:corresponding Aut

• FUJIWARA Masayuki

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• OSAKI Takeshi

  Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

• Kanamori Takeshi

  Graduate School Of Agriculture And Biological Sciences Osaka Prefecture University

• Osaki Takeshi

  Graduate School Of Agriculture And Biological Sciences Osaka Prefecture University

• Fujiwara Masayuki

  Graduate School Of Agriculture And Biological Sciences Osaka Prefecture University

• FUJIWARA Masayuki

  Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

関連論文

• The Putative RNA-Processing Protein, THO2, is a Microtubule-Associated Protein in Tobacco
• Amino Acid 129 of Cucumber mosaic virus Coat Protein Determines Local Symptom Expression and Systemic Movement in Tetragonia expansa, Momordica charantia and Physalis floridana
• Effect of Figaren, an Antiviral Protein from Cucumis figarei, on Cucumber mosaic virus Infection
• Movement of Cucumber Mosaic Virus Is Restricted at the Interface between Mesophyll and Phloem Pathway in Cucumis figarei
• Nucleotide Sequence Analysis for RNA 3 of pepo-, SO- and MY17-Strains of Cucumber Mosaic Virus and its Comparison with Y-Strain
• Purification and Partial Characterization of Figaren, an RNase-like Novel Antiviral Protein from Cucumis figarei
• Cloning and Analysis of a cDNA Coding a Putative Ribosome-Inactivating Protein from Cucumis figarei

スポンサーリンク